Journal: Experimental and Therapeutic Medicine

Article Title: Effect of autophagy-associated proteins on the arecoline-induced liver injury in mice

doi: 10.3892/etm.2018.6564

Figure Lengend Snippet: Expression of beclin 1 determined by immunohistochemistry. (A) Beclin 1 expression was weak in normal control liver cells. (B) Model group. (C) Low-dose glutathione group. (D) high-dose glutathione group (Original magnification, ×400; black arrows indicate positive staining). Beclin 1 expression was strong in all drug-treated groups. (E) On day 15, beclin 1 expression was significantly higher in glutathione-administrated groups than control group and model group. On day 30, beclin 1 expression increased in all drug-administrated groups compared with control group. *P<0.05 vs. Control group; Δ P<0.05. vs. model group.

Article Snippet: Each sample was pretreated with normal goat serum (OriGene Technologies, Inc.) for 30 min at room temperature to block nonspecific binding and incubated with rabbit anti-mTOR (diluted 1:800), rabbit anti-beclin 1 (diluted 1:120) or rabbit anti-MAP1LC3B (diluted 1:60) antibodies overnight at 4°C.

Techniques: Expressing, Immunohistochemistry, Staining

Journal: Experimental and Therapeutic Medicine

Article Title: Effect of autophagy-associated proteins on the arecoline-induced liver injury in mice

doi: 10.3892/etm.2018.6564

Figure Lengend Snippet: Expression of mTOR, beclin 1, MAP1LC3B and caspase-3 determined by western blotting. The results indicated that mTOR expression decreased in all drug-treated groups compared with the control. The expression of beclin1, MAP1LC3B and caspase-3 increased in all drug-treated groups compared with the respective control groups. *P<0.05 vs. Control group. mTOR, mammalian target of rapamycin; MAP1LC3B, protein microtubule associated protein 1 light chain 3 B.

Article Snippet: Each sample was pretreated with normal goat serum (OriGene Technologies, Inc.) for 30 min at room temperature to block nonspecific binding and incubated with rabbit anti-mTOR (diluted 1:800), rabbit anti-beclin 1 (diluted 1:120) or rabbit anti-MAP1LC3B (diluted 1:60) antibodies overnight at 4°C.

Techniques: Expressing, Western Blot

Journal: Molecular cell

Article Title: Phosphoglycerate kinase 1 phosphorylates Beclin1 to induce autophagy

doi: 10.1016/j.molcel.2017.01.027

Figure Lengend Snippet: (A) 1 × 105 U87 cells, with or without PGK1 or Beclin1 depletion and reconstituted expression of the indicated proteins, were cultured in hypoxia condition for 72 h. These cells were collected and counted. Data represent the mean ± SD of quadruplicate samples. ** P < 0.001.

Article Snippet: Rabbit polyclonal antibodies against PGK1 K388 acetylation and Beclin1 S30 phosphorylation were custom produced by Signalway Antibody (College Park, MD).

Techniques: Expressing, Cell Culture